Wayne Moss, a prolific Nashville guitarist who helped broaden the country sound through experimental country-rock groups Area Code 615 and Barefoot Jerry, died Monday at his home in Madison, Tenn. He was 88. The West Virginia native was one of three guitarists who played the iconic staccato riff on Roy Orbison’s “Oh, Pretty Woman,” a chart-topping pop single in 1964.

A member of Nashville’s elite A-Team of first-call studio musicians, Mr. Moss played on recordings by luminaries including Patsy Cline, Loretta Lynn, Charley Pride and Tammy Wynette. He improvised the filigreed guitar phrasing on Bob Dylan’s “I Want You” and the atmospheric part on Dolly Parton’s “Jolene.” He also played on “Coat of Many Colors” and “I Will Always Love You,” as well as Wynette’s crossover hit “Stand By Your Man.”

In 1969, Mr. Moss formed Area Code 615, named for Nashville’s area code, and two years later started Barefoot Jerry, an improvisational Southern rock band that released six albums in the 1970s. He built Cinderella Sound studio in 1961 in a converted two-car garage outside his home, which became perhaps Nashville’s oldest continuously operating independent sound studio, hosting projects by Linda Ronstadt and the Steve Miller Band. Despite never learning to read music, he helped develop the Nashville number system used by studio musicians. His survivors include his wife and five children from earlier marriages.